DIAGNOSIS OF KAPOSI'S SARCOMA-ASSOCIATED HERPESVIRUS (KSHV) INFECTION

摘要

The present invention relates to methods and compositions for diagnosis of KSHV. In particular, this invention relates to production of KSHV viral proteins and peptides in mammalian cells, purification of KSHV viral proteins and polypeptides from KSHV-infected cells in culture and their utility in detecting serum antibodies. K8.1 and LANAl produced in mammalian cells and purified under non-denaturing conditions are vastly superior to previously used recombinant antigens and are key ingredients of high sensitivity and specificity assay to detect KSHV infection.