IN VITRO CULTURE METHOD FOR MYCOBACTERIUM LEPRAE

摘要

The object is to establish a method for establishing a cell line having low proliferation ability and low-temperature resistance, and to provide a simple and long-term in vitro culture method for Mycobacterium leprae. Disclosed is a method for establishing a low-temperature-resistant, (very-)low-proliferative cell line, which is characterized by culturing a tumor cell produced from a human hemangiosarcoma subcutaneously transplantable into an SCID (severe combined immunodeficiency disease) mouse in a culture medium containing a mouse hemangiosarcoma cell line ISOS-1 culture supernatant, selecting a cell that is not detached by the trypsin-EDTA treatment from cultured cells, and subculturing the cell line while reducing the amount of the ISOS-1 culture supernatant to be added to the culture medium. Also disclosed is a cell line produced by the method. Further disclosed is a culture method for Mycobacterium leprae.