THERMOPHILIC LONG CHAIN ALKANOL DEHYDROGENASE AND ITS ENCODING GENE AND USES THEREOF

摘要

An alcohol dehydrogenase, which is isolated from Geobacillus thermodenitrificans NG80-2, consists of 387 amino acids shown in SEQ ID NO: 2. The enzyme has ability to degrade long chain alkanol of chain length longer than C16. It can be widely act on long chain alkanol of chain length between C12 and C30, and optimum substrate is octyl alcohol. A gene encoding the enzyme, a recombinant plasmid pET-28a (+) expressing the enzyme, and a recombinant Escherichia coli BL21 producing the same are further provided.