HYBRIDIZATION METHOD BETWEEN LEUCINE RICH REPEAT FAMILY PROTEINS

摘要

Disclosed is a method for fusing proteins. More specifically, disclosed is a method of fusion between LRR (Leucine Rich Repeat )-famiIy proteins, the method comprising fusing the LRR-family proteins with each other at the following consensus sequence position: LxxLxxLxLxxN, wherein L represents alanine, glycine, phenylalanine, tyrosine, isoleucine, valine or tryptophane, N represents asparagine, glutamine, serine, cysteine or threonine, and X represents any amino acid. According to the disclosed fusion LRR technique, a fusion protein having high crystallinity can be obtained by fusing an LRR-family protein having high crystallinity with another LRR-family protein having low crystallinity. Thus, the fusion LRR technique is useful to determine the structures of proteins having low crystallinity. Furthermore, because the active regions and mechanisms of proteins can be understood from the structural analysis of the proteins, the fusion LRR technique can be effectively to understand the in vivo action of the proteins and develop therapeutic drugs. In addition, according to the fusion LRR technique of the present invention, fusion proteins, which have high crystallinity while maintaining the function of the respective proteins, can be produced in large amounts. Thus, the fusion LRR technique is useful for the development of protein drugs.