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PURIFICATION OF PROTEINS BY AFFINITY PRECIPITATION WITH STIMULI RESPONSIVE POLYMERS
PURIFICATION OF PROTEINS BY AFFINITY PRECIPITATION WITH STIMULI RESPONSIVE POLYMERS
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机译:通过亲和响应聚合物的亲和沉淀纯化蛋白质。
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摘要
The present invention relates to a selectively soluble polymer capable of binding to a desired molecules in an unclarified mixture containing various biological materials and the methods of using such a polymer to purify a molecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired molecule (protein, polypeptide, etc) and remains capable of binding to that molecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.
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