RECONSTRUCTION AND VISUALIZATION OF NEURONAL CELL STRUCTURES WITH BRIGH-FIELD MOSAIC MICROSCOPY

摘要

A reconstruction method to reconstruct an image of a neuronal cell structure from a recorded image comprising an image stack of image layers recorded with a bright field microscope, is described which comprises the steps of deconvolution of the recorded image for providing a corrected image, and extraction of a cell structure image from the corrected image, the deconvolution comprises a linear deconvolution being applied to the image stack on the basis of a point spread function of the bright field microscope, which point spread function is calculated on the basis of measured features of the bright field microscope. Furthermore, an imaging method including the reconstruction method, and reconstruction and imaging devices are described.