Production of transgenic Panax ginseng hairy roots using the activation tagging system

摘要

PURPOSE: A production of method transgenic Panax Ginseng is provided, thereby rapidly and easily obtaining a library of the total mutant genome of Panax Ginseng. CONSTITUTION: A production method of transgenic Panax Ginseng comprises the steps of: (A) constructing an activation tagging vector containing an enhancer and a selection marker; (B) inserting the activation tagging vector into A. rhizogenes; (C) transforming Panax Ginseng with A. rhizogenes containing the vector; and (D) culturing the A. rhizogenes treated Panax Ginseng in a selection medium to select a transgenic Panax Ginseng, wherein the enhancer is CaMV 35S.