Two kinds of nuclear ribonucleotide protein present in the sample B1 (hnRNP B1) terminal "3 from the end of homology to at least a portion of the downstream first primer and the specific nucleotide sequence" as the method of measuring the mRNA, the 5, of a specific nucleotide sequence of the RNA from complementary claim to at least a portion of the upstream-2 primer 2 containing the (at least one has a 5 'promoter sequence to the ends of the first and second primer) to the specific nucleotide sequence to the promoter sequence downstream of the promoter sequence by process for generating a single-stranded DNA, a step of generating the RNA transcription product by the double-stranded DNA as a template, is the template for the RNA transcription product is continue the DNA synthesized by the process, more than each of the step of generating the double-stranded DNA nucleic acid amplification process in which the process is repeated at conditions which proceeds at the same time and comprises a step of measuring the amount of the RNA transcription product. ; hnRNP B1, mRNA measurement, cancer diagnosis
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