Antibody and hapten-protein conjugate utilized in an enzyme-linked immunosorbent assay (ELISA) for herbicide metamifop residues

摘要

A conjugate of a hapten compound of metamifop and a protein is provided to be used as an antibody for rapidly and quantitatively detecting the metamifop. A conjugate is characterized in that a hapten compound of metamifop is bound to a protein by an amide bond. The hapten compound of the metamifop is represented by the formula(1), wherein each R1, R2, R3, and R4 is independently H, carboxyl, hydroxy, C1-10 alkyl, halogen, cyano, amino, thiol, aldehyde, nitro, C2-10 carboxyalkyl or C1-10 hydroxyalkyl; each R5 and R6 is independently H, carboxyl, hydroxy, C1-10 alkyl, halogen, cyano, amino, thiol, aldehyde, nitro, C1-10 carboxyalkyl, C1-10 hydroxyalkyl or a group represented by the structural formula(1-a); R7 is H, C2-10 carboxyalkyl or C1-10 alkyl; and each R8, R9, R10, R11 and R12 is independently H, carboxyl, hydroxy, C1-10 alkyl, halogen, amino, thiol, aldehyde, C2-10 carboxyalkyl or C1-10 hydroxyalkyl, provided that at least one of the R1 to R12 is at least one of carboxyl and amino. The protein is bovine serum albumine(BSA), human serum albumine(HSA), fibrinogen, ovalbumin, thyroglobulin or keyhole limpet hemocyanin(KLH). An antibody is produced by being reacted to the conjugate and is specifically bound to the metamifop. A method for producing the antibody comprises the steps of: (a) immunizing mammals with the conjugate; and (b) selecting the antibody specific to the metamifop from the mammals.