PRODUCTION OF L-CYSTEINE USING HIGH SUBSTRATE CONCENTRATIONBIOCONVERSION

摘要

A method of manufacturing high concentration of L-cysteine biologically is provided to manufacture the high concentration of L-cysteine by adding aiding agent and salt contributing to inducible enzyme stabilization in a process of obtaining the eutectic base compound and lowering a melting point. A method of manufacturing high concentration of L-cysteine biologically comprises steps of: mixing D,L-2-amino-Delta2-thiazoline-4-carboxylic acid, D-sorbitol, Na2CO3, MgCl2 mixture and melting aiding agent of tris hydrochloride buffered aqueous solution and obtaining transparent eutectic base compound; and putting enzyme mixture of fungi including L-ATC hydrolase and S-carbamyl-L-cystein hydrolase in the eutectic base compound, reacting enzyme and obtaining the L-cysteine. The fungi is Zoogloea species.