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DNA SELECTION AND DETECTION METHOD USING PROTEINS BINDING SPECIFIC DNA BASE SEQUENCES
DNA SELECTION AND DETECTION METHOD USING PROTEINS BINDING SPECIFIC DNA BASE SEQUENCES
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机译:结合特定DNA碱基序列的蛋白质的DNA选择和检测方法
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摘要
Provided is a method for selectively detecting DNA by using a protein specifically coupled with DNA base sequence in no need of using single strand DNA or a fluorescent label in DNA hybridization. A method for selectively detecting DNA comprises the following steps of: binding fluorescent particles to a limitation enzyme of sequence specific DNA-binding protein; reacting the limitation enzyme to a DNA sample; and analyzing the limitation enzyme-bound DNA. The fluorescent particle is selected from the group consisting of a quantum dot, self- fluorescent particle, and a nano-sized fluorescent particle.
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