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CELL-BASED REPORTER SYSTEM FOR SCREENING NATURAL PRODUCTS REACTIVATING GAMMAHERPESVIRUS AND SCREENING METHOD USING THE SAME
CELL-BASED REPORTER SYSTEM FOR SCREENING NATURAL PRODUCTS REACTIVATING GAMMAHERPESVIRUS AND SCREENING METHOD USING THE SAME
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机译:基于细胞的报告员系统,用于筛查活化革藻病毒的天然产物和使用该方法的筛查方法
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摘要
A reporter system for searching a natural compound reactivating gamma herpesvirus in cell line infected with gamma-herpesvirus is provided to help activation of anti-herpes agent and search natural compound without toxicity. A reporter cell line for searching an inducing material of reactivating gamma herpes virus comprises: a PAN RNA (polyadenylated nuclear RNA) promoter (sequence number 1[SEQ ID NO:1]) of Kaposi's sarcoma-associated herpesvirus (KSHV); and a reporter gene which is connected to be expressed by a promoter. The reporter gene is destabilized enhanced green fluorescent protein (EGFP). The gamma herpes virus is a cell line which is infected with KSHV or Epstein-Barr virus (EBV).
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机译:本发明提供了一种用于在感染了γ-疱疹病毒的细胞系中搜索活化γ-疱疹病毒的天然化合物的报道系统,以帮助抗疱疹剂的活化并搜索没有毒性的天然化合物。用于寻找活化γ疱疹病毒的诱导材料的报道细胞系包括:卡波西氏肉瘤相关疱疹病毒(KSHV)的PAN RNA(聚腺苷酸核RNA)启动子(序列号1 [SEQ ID NO:1]);和连接到由启动子表达的报道基因。报道基因是不稳定的增强型绿色荧光蛋白(EGFP)。伽马疱疹病毒是感染了KSHV或爱泼斯坦-巴尔病毒(EBV)的细胞系。
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