Genome­scale Metabolic Network Model in Butanol­producing Microorganism and Method for Analyzing Metabolic Feature and for Screening Knock­out Targets in Butanol­producing Microorganism Using the Same

摘要

PURPOSE: A network model of microorganism metabolism, metabolic property of butanol-producing microorganism or a method for screening deletion target is provided to predict genetic deletion target in a short time. CONSTITUTION: An analysis of metabolism of microorganism which produces buthanol using metabolism network model or a method for screening deletion target comprises a step of collecting genome information or information relating to each gene and a step of arranging enzyme reaction formulation in C. acetobutylicum and GPR relation. The enzyme relating to enzyme reaction is alcohol dehydrogenase, butanol dehydratase, butyryl coenzyme A dehydratase, acetylcoenzyme-A transacetylase, 3-hydroxybutyril coenzyme A dehydratase, and hydrogenase.