ANTIBODY PHAGE SURFACE DISPLAY LIBRARY AND PHAGEMID VECTOR CONTAINING ANTIBODY PHAGE SURFACE DISPLAY LIBRARY GENE

摘要

PURPOSE: A method for producing an antibody phage display library and a phagemid vector containing an antibody phage display library gene are provided to ensure high availability in developing a novel drug and produce synthesis human antibody library of high quality.;CONSTITUTION: A method for producing an antibody phage surface display library comprises: a step of introducing restriction enzyme cutting sequence to produce pFDV plasmid vector; a step of cutting the pFDV plasmid vector; a step of inserting scFv gene library; a step of culturing the pFDV vector in a medium containing antibiotics; a step of purifying and isolating pFDV plasmid vector; a step of performing PCR and transplating CDR-H3; and a step of cutting CDR-H3 transplanted scFv library gene and pComb3X vector with each restriction enzyme, ligating, and culturing.;COPYRIGHT KIPO 2010