METHOD FOR PREPARING FOSMID LIBRARY OF PIERIS RAPAE FOR ANALYSIS OF GENE SEQUENCE

摘要

PURPOSE: A method for preparing fosmid library of Pieris rapae is provided to reduce time and cost for sequence analysis.;CONSTITUTION: A method for preparing fosmid library of Pieris rapae comprises: a step of completely pulverized with a tissuelizer; a step of adding 20ml of suspension to 1,250g of pulverized sample; a step of centrifuging the completely suspended sample at 5000rpm and 4°C for 10 minutes to remove debris; and a step of isolating nucleus.;COPYRIGHT KIPO 2010