METHOD FOR MODIFYING TEMPLATE TO ANALYZE MULTIPLE MRNA OR 16S RRNA BY CAPILLARY ELECTROPHORESIS AND DETECTING MICROORGANISM BY USING THE SAME

摘要

PURPOSE: A method for modifying a template for analysis multiple mRNA or 16S rRNA is provided to detect microorganism by shortening analysis time and enhance analysis sensitivity and accuracy. CONSTITUTION: A method for modifying a template for analysis of multiple mRNA or 16S rRNA comprises: a step of performing reverse transcriptase PCR with a primer containing template-specific sequence and overhang sequence of mRNA or 18S rRNA to prepare cDNA having universal base sequence; a step of constructing template switching primer; and a step of performing PCR with the primer to obtain double strand DNA template with common sequence at both terminal of the sequence.