16 Method for modifying template to analyze multiple mRNA or 16S rRNA by capillary electrophoresis and detecting microorganism by using the same

摘要

The present invention relates to a template modification method for analyzing multiple mRNA or 16S rRNA by capillary electrophoresis and a method for detecting microorganisms using the same. More particularly, the present invention relates to a method for detecting a mRNA or rRNA, A modified double stranded DNA template having a common nucleotide sequence on the front and back sides was prepared, PCR amplified double stranded DNA template using a common primer, and the amplified product was subjected to a capillary electrophoresis-single strand conformation polymorphism strand conformation polymorphism), which has a high analytical sensitivity and accuracy and a shortening of analysis time, and a template for analysis of multiple mRNA or 16S rRNA by capillary electrophoresis which can detect microorganisms using the same And a method for detecting microorganisms using the same.;RNA, template switching, modification, capillary electrophoresis