Method for producing bio-diesel in supercritical fluid condition using lipase

摘要

A method for producing bio-diesel is provided to shorten reaction time and obtain the bio-diesel with high yield using lipase in supercritical fluid condition during an enzymatic process. In a method for producing bio-diesel using oils and fats and alcohol as substrates and lipase through an enzymatic process, the enzymatic process is performed in supercritical fluid condition under the pressure of 100-150 bar at a temperature of 40-60 deg.C at a stirring speed of 100-300 rpm, wherein the lipase uses 1,3-position specific lipase and a non-position specific lipase in a weight ratio of 1:3-3:1. Further, a supercritical fluid is a supercritical carbon dioxide.