Method, Polynucleotide Probe, DNA Chip and Kit for Identifying Mutation of Human Mitochondrial DNA

摘要

The present invention relates to extracting the DNA polynucleotide probe to determine mutations of the human mitochondrial DNA and the method determine variations, DNA chip and kit, in particular from human blood, cells or tissues; To obtain a PCR product by performing a polymerase chain reaction (PCR) for the extracted DNA; The PCR product with SEQ ID NO: 5 to SEQ ID NO: hybridization step solidifying the same as any one of the polynucleotide probes of DNA sequences, or 90 comprising a complementary (complementary) nucleotide sequence; And if depend on mutation detection method of the human mitochondrial DNA to determine whether the exchange goods comprising the step of determining the variation with which the human mitochondrial DNA, variation in the number of human mitochondrial DNA (mutation) and the faster the mitochondrial haplotype (haplotype) of the DNA on a slide, can be accurate inspection. ; Human mitochondria, single nucleotide polymorphisms, mutations