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Adjuvants Including The Lytic Extract of Streptomyces Albus
Adjuvants Including The Lytic Extract of Streptomyces Albus
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机译:佐剂包括链霉菌的溶菌提取物
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摘要
An immunostimulant containing the lystic extract of Streptomyces albus is provided to reinforce the functions of various immune cells having the major role in the disease defense, thereby increasing the specific and nonspecific resistance to the disease infection of animals. The immunostimulant contains the lystic extract of Streptomyces albus which is prepared by cultivating Streptomyces albus in yeast malt extract agar broth at 26 deg. C for 72 hours, centrifuging the cultured fluid at 3000Xg for 30 minutes and collecting it, rinsing the collected pellet with phosphate buffered saline(PBS) of pH 7.2 and suspending the rinsed pellet in PBS, adding 5% of TNT solution containing 50mM of Tris-HCl, 0.15M of NaCl and 4% Triton x-114 as solubilizing agent into the Streptomyces albus suspension, stirring the mixture at room temperature for 2-3 days to solubilize the Streptomyces albus, and regulating pH of the solution to 6.5-7.2, and drying the solution in the thermostatic chamber.
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