Wednesday for virus isolation, its use and kit for the diagnosis of viruses, INCLUDING THIS WEDNESDAY

摘要

1. wednesday u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus, u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 wednesday u0434u043bu00a0 cell culture with addition of at least one hormone and at least one enzyme.;2. wednesday u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus in 1, where the hormone u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a glucocorticoid hormone.;3. wednesday u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus in 2, where the hormone is selected from dexamethasone, of hydrocortisone. i.v. push, cortisone acetate and prednisone, prednisolone, u043cu0435u0442u0438u043bu043fu0440u0435u0434u043du0438u0437u043eu043bu043eu043du0430, betamethasone and, u0442u0440u0438u0430u043cu0446u0438u043du043eu043bu043eu043du0430, beclometasone, u0444u043bu0443u0434u0440u043eu043au043eu0440u0442u0438u0437u043eu043du0430 acetate, u0434u0435u0437u043eu043au0441u0438u043au043eu0440u0442u0438u043au043eu0441u0442u0435u0440u043eu043du0430 acetate (DOCA) and u0430u043bu044cu0434u043eu0441u0442u0435u0440u043eu043du0430.;4. wednesday u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus on paragraph 3, where an u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 hormone dexamethasone.;5. wednesday u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus in 1, where the enzyme u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a u0441u0435u0440u0438u043du043eu0432u0443u044e or u0430u0441u043fu0430u0440u0442u0430u0442u043du0443u044e u043fu0440u043eu0442u0435u0430u0437u0443.;6. wednesday u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus on clause 5, where the enzyme is selected from the u0442u0440u0438u043fu0441u0438u043du0430, u0445u0438u043cu043eu0442u0440u0438u043fu0441u0438u043du0430 or u043fu0435u043fu0441u0438u043du0430.;7. wednesday u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus for p.6, where the enzyme u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a trypsin.;8. wednesday u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus in 1, where the enzyme is present in a quantity of from 1 to 5 micrograms / ml, especially about 2.5 micrograms / ml.;9. wednesday u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus in 1, where the hormone is present in a concentration of 10 - 10 4m to 6m, especially around 10 - 5m.;10. method u043eu0431u043du0430u0440u0443u0436u0435u043du0438u00a0 virus, including;(1) the cell lines suitable u0434u043bu00a0 u0438u043du043eu043au0443u043bu00a0u0446u0438u0438 virus;;(2) specific preliminary processing sample to obtain sample that potentially contains a virus, which is detectable;;(3) u0438u043du043eu043au0443u043bu0438u0440u043eu0432u0430u043du0438u0435 cells sample that potentially contains a virus, which is detectable;;(4) u0438u043du043au0443u0431u0430u0446u0438u044e u0438u043du043eu043au0443u043bu0438u0440u043eu0432u0430u043du043du044bu0445 cells;;(5) the replacement of environment u0434u043bu00a0 sample environment u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus containing environment u0434u043bu00a0 cell culture with addition of at least one hormone and at least is alone wow enzyme;;(6) u0438u043du043au0443u0431u0430u0446u0438u044e model of (4); and;(7) the discovery of the virus.;11. way on p.10, where the hormone u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a glucocorticoid hormone.;12. method for the p.11, where the hormone is selected from dexamethasone, of hydrocortisone. i.v. push, cortisone acetate and prednisone, prednisolone, u043cu0435u0442u0438u043bu043fu0440u0435u0434u043du0438u0437u043eu043bu043eu043du0430, u0431u0435u0442u0430u043cu0435u0442u0430u0437u043eu043du0430, u0442u0440u0438u0430u043cu0446u0438u043du043eu043bu043eu043du0430, beh u043au043bu043eu043cu0435u0442u0430u0437u043eu043du0430, u0444u043bu0443u0434u0440u043eu043au043eu0440u0442u0438u0437u043eu043du0430 acetate, u0434u0435u0437u043eu043au0441u0438u043au043eu0440u0442u0438u043au043eu0441u0442u0435u0440u043eu043du0430 acetate (DOCA) and u0430u043bu044cu0434u043eu0441u0442u0435u0440u043eu043du0430.;13. way on p.12, where a u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 hormone dexamethasone.;14. method for enzyme u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 p.10, where a u0441u0435u0440u0438u043du043eu0432u0443u044e or u0430u0441u043fu0430u0440u0442u0430u0442u043du0443u044e u043fu0440u043eu0442u0435u0430u0437u0443.;15. a way to question 14, where the enzyme is selected from the u0442u0440u0438u043fu0441u0438u043du0430, u0445u0438u043cu043eu0442u0440u0438u043fu0441u0438u043du0430 or u043fu0435u043fu0441u0438u043du0430.;16. method u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus on p.15, where the enzyme u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a trypsin.;17. method u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus on p.10, where the enzyme is present in a quantity of from 1 to 5 micrograms / ml, especially about 2.5 micrograms / ml.;18. method u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus on p.10, where the hormone is present in a concentration of 10 - 10 4m to 6m, especially around 10 - 5m.;19. a set of u0434u043bu00a0 diagnosis of the virus, including:;(1) the environment u0434u043bu00a0 u0432u044bu0434u0435u043bu0435u043du0438u00a0 virus containing the environment u0434u043bu00a0 cell culture with addition of at least one hormone and at least one enzyme.;(2) a set of u043cu0438u043au0440u043eu0442u0438u0442u0440u043eu0432u0430u043bu044cu043du044bu0445 next.;20. a set of u0434u043bu00a0 diagnosis virus on p.19, where a set of u043cu0438u043au0440u043eu0442u0438u0442u0440u043eu0432u0430u043bu044cu043du044bu0445 and contains many united, but u043eu0442u0434u0435u043bu00a0u0435u043cu044bu0445 u043cu0438u043au0440u043eu0442u0438u0442u0440u043eu0432u0430u043bu044cu043du044bu0445 next.