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METHOD FOR PRODUCING A SERUM FOR DIAGNOSIS anaplasmosis of cattle in the indirect immunofluorescence BY hyperimmunization RABBITS
METHOD FOR PRODUCING A SERUM FOR DIAGNOSIS anaplasmosis of cattle in the indirect immunofluorescence BY hyperimmunization RABBITS
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机译:超免疫RABBITS间接免疫荧光法生产牛诊断性血清病的方法
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摘要
FIELD: veterinary science.;SUBSTANCE: method pertains to laboratory diagnostic methods of anaplasmosis and may be used in veterinary. Method consists in injecting of antigen and levamisole immunostimulant to the rabbits. Whereat as an antigen an antigen derived by alternating freezing and thawing of the grown on the Vero cells in the growth supporting nutrient medium "Igla" MEM with added embryonic serum, amino acids and antibiotics of vial culture Anaplasma sp. Omsk after the eight days of incubation.;EFFECT: method enables getting anaplasmosis serum with high antibodies diagnostic titer.;5 tbl
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