A method for isolating and differentiating Vibrio mimicus PHAGE

摘要

A method of isolating and differentiating phage Vibrio mimicus, comprising incubating a lysogenic strain and use of the indicator strain, characterized in that as the indicator strain used Vibrio cholerae eltor KM 199, which is a three-hour broth culture of 02 ml is introduced into the mixture incubated filtered lysogenic organism 1 ml of the latter is connected to the 5 ml of 0.7% agar Martin, then the slurry was plated on agar plate of 1.5% agar Martin, cultured at 37 ° C for 24-48 hours and the lytic reaction concludes that there and phage Vibrio mimicus F-19, with its differentiation by phage FB cholera O139 serogroup is carried out using the strain Vibrio cholerae 1322-037 serovar, to prepare 0.2 ml of four-hour broth culture of the latter, is added 5 ml of 0.7% agar and poured on an agar plate in a Petri dish, after curing is applied on one track phage F-19, and the second - Vibrio phage FB is placed in the incubator and were grown for 24-48 hours at a temperature of 37 ° C, for register lysis at one of the tracks confirm the presence on it of the phage Vibrio mimicus.