METHODS FOR MAKING POLYCLONAL ANTIBODIES, ANTISERUM CONTAINING POLYCLONAL ANTIBODIES, AND METHODS FOR IMMUNISATION

摘要

FIELD: medicine.;SUBSTANCE: method for making polyclonal Nogo protein antibodies by protein immunisation of an animal, with at least 6 amino-acid residues of active area Nogo A, free from any other myelin material of central nervous system whereto bound in natural conditions. Said active area covers position 1-171, 260-974, 1163-1178 of the corresponding amino acid sequence of protein Nogo A. There is disclosed separated antiserum based on polyclonal antibodies made under said method. There are disclosed methods for immunisation of a non-human animal to make polyclonal antibodies, as well as method for making monoclonal antibody, and the specified monoclonal antibody.;EFFECT: making active protein Nogo A antibodies to be applied in medicine for neurite growth activation.;26 cl, 18 dwg, 2 tbl, 8 ex