METHOD OF OBTAINING PREPARATION BASED ON MICROFUNGI CHLAMYDOSPORES FOR VEGETATION AND ANIMAL PARASITIC NEMATODE CONTROL

摘要

FIELD: biotechnology.;SUBSTANCE: invention concerns biotechnology, particularly methods of obtaining preparation based on chlamydospores of nematophagous microfungus for vegetation and animal parasitic nematode control. Method involves submerged cultivation of microfungus in nutritive medium including carbon and organic nitrogen sources, salts and water, till maximum accumulation of vegetative mycelial mass, introduction of sporulation inducer, with further chlamydospore formation. Duddingtonia flagrans F-882 is used as microfungus, submerged cultivation is performed in gas vortex bioreactor until dry rate of biomass reaches at least 10.0 g/l. To obtain fluid form of preparation, water is added as sporulation inducer, diluting vegetative mycelial mass by 2-5 times, and/or glycerin is added in end concentration of 1-2 wt %, and additionally the biomass is incubated until chlamydospore accumulation in the culture reaches maximum. To obtain dry form of preparation, sterile foamed vermiculite is added to vegetative mycelial mass as sporulation inducer at the ratio of 1.0-4.0 ml of mycelial mass to 1 g of foamed vermiculite, obtained mix is stirred and additionally incubated to obtain chlamydospores.;EFFECT: enhanced efficiency of preparation.;6 cl, 10 tbl, 11 ex