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A method for the parallel isolation of double- and single-stranded nucleic acids and for the selective removal of double-stranded nucleic acids from a mixture of double-stranded and single-stranded nucleic acids
A method for the parallel isolation of double- and single-stranded nucleic acids and for the selective removal of double-stranded nucleic acids from a mixture of double-stranded and single-stranded nucleic acids
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机译:一种双链和单链核酸的平行分离方法,以及从双链和单链核酸的混合物中选择性去除双链核酸的方法
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摘要
A method for i) parallel isolation of a double-stranded and/or a single-stranded nucleic acid and/or ii) selective removal of a double-stranded nucleic acid from a mixture of a double-stranded and a single-stranded nucleic acid or from a source comprising a double-stranded and a single-stranded nucleic acid includes absorbing the double-stranded nucleic acid onto a first solid carrier, while the single-stranded nucleic acid is not adsorbed and remains in solution, removing the first carrier with the adsorbed nucleic acid from the solution, mixing the solution comprising the single-stranded nucleic acid with an alcoholic solution having a concentration of 1 to 90 vol.-%, and contacting the resulting solution with second solid carrier, to absorb the single-stranded nucleic acid onto the second solid carrier.
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