Method and immune absorbents for the specific detection and absorption of celiac disease - and dermatitis herpetiformis associated antibody

摘要

The invention relates to a method and reagents for serological diagnosis of celiac disease and / or the dermatitis herpetiformis by the detection antibody directed against modified gliadin as well as a method and pharmaceutical compositions for the treatment of these diseases by specific immunoabsorption this antibody. The invention relates to a method for improving the bonding of a particularly with two diseases associated fraction of antibodies against gliadin or gliadin analog synthetic peptides, as a result of which the diagnosis and therapy of celiac disease is improved and dermatitis herpetiformis. In a particular embodiment, an immunologically dominant, deamidated epitope of the gliadins, consisting of nine amino acids, linked to an artificial gliadin - homologous octapeptide, to increase the diagnostic competence for the detection of celiac disease - associated antibody, and for an improved efficiency of their elimination by means of immunoabsorption used from the patient's blood.The present invention is based on artificially created peptides, which or have been synthesized chemically and, instead of the produced from the prior art known monomers individual peptides or peptide mixtures in the context of immune tests, or for separating off against gliadin - analogous peptides can be used antibody directed. By means of the manufacture of a synthetic gliadin - analog polypeptide, which the diagnostic advantages of the state ..