device and procedure for the collection of gerinnungsfunktionen global, especially primary haemostasis

摘要

Apparatus for investigating e.g. primary hemostasis comprises a feed chamber (2) and a reaction chamber (39) with a flow channel, where blood is fed through the channel by a piston and cylinder unit (1) and a pressure sensor (9) measures variations in pressure in a blood-free pressure measuring chamber (3), which are dependent on the reactions taking place in the reaction chamber. Apparatus for investigating global, especially primary hemostasis in whole blood or platelet-enriched plasma comprises a feed chamber (2) and a reaction chamber (39) with a flow channel for the blood. The blood is fed through this by a piston and cylinder unit (1). A pressure sensor (9) measures variations in pressure in a blood-free pressure measuring chamber (3), which are dependent on the reactions taking place in the reaction chamber. Blood which has passed through the reaction chamber is then fed to a collecting vessel. The pressure measuring chamber is positioned below the feed chamber and above the surface of the blood passing through the reaction chamber into the collecting vessel. An independent claim is also included for a method for investigating global, especially primary hemostasis in whole blood or platelet-enriched plasma in which blood is fed from a feed chamber through an orifice into a reaction chamber under predetermined shear or flow conditions. It is then fed on to a reaction surface where coagulation takes place (e.g. clot formation under the influence of thrombin), forming a structure composed of polymerized fibrin and cellular components (thrombocytes, erythrocytes and leukocytes) or a force is developed which is exerted by activated thrombocytes on the fibrin network in the reaction orifice. The alteration in pressure due to this is then measured. The shear rate, shear force or flow rate is then adjusted, depending on the measured pressure or blood viscosity calculated from it, to conform to a predetermined value.