determination of human cytochrome p450 3a4 (cyp3a4)

摘要

Monoclonal antibodies (MAbs) have been developed to human cytochrome P450 2C8/9 (CYP2C8/9) by the fusion of myeloma tumour cells with isolated B-lymphocytes from spleens of mice immunised with baculovirus-expressed human CYP2C9; the MAbs for CYP2C9 are very powerful inhibitors of CYP2C8/9 activity and stray binders of the enzymes on Western blot and exhibit no cross-reactivity towards any of the other P450 subfamily members; based on these features the MAbs are useful tools for measuring CYP2C8/9 and assessing the role of CY2C8/9 in the metabolism of various compounds.