New terthiophene - 3 - carboxylic acid compound and a method for their preparation, functionalised conductive terthiophenpolymer with the compound as a monomer and a method for the detection of dna - hybridization under application of the polymer

摘要

Disclosed are a novel terthiophene-3-carboxylic acid compound, a functionalized conductive terthiophene polymer having carboxylic groups produced in an electrochemical method using the compound as a monomer, and a novel process for detecting DNA hydridization through impedance measurement using the polymer. In addition, a method for manufacturing a probe DNA used in the process for detecting DNA hybridization is provided. Since the process can detect DNA hybridization using impedance change, without the use of any indicator, small sensor systems which measure impedance in a particular frequency range can be fabricated. The systems are applicable to portable sensors for identifying DNA sequence hybridization for clinical examination and disease diagnosis. Further, the proces has an excellent selectivity of complementary sequences to mismatched sequences.