Diagnosing infection, SIRS or sepsis using RT-PCR and biomarkers

摘要

A method for screening a biological sample to detect early stages of infection, SIRS or sepsis comprising the steps of detecting expression of at least one biomarker from a first set and one from a second set of informative biomarkers by RT-PCR; analysing the results of detection; classifying said sample according to the likelihood and/or timing of the development of overt infection. The first set of biomarkers consists of CD40, CD5, CD79A, CRX, CTNND1, CX3CL1, ENTPD2, ENTPD5, EPHA8, GPR44, HMMR, IL8, MAP1A, MAPK7, MEF2D, ODF1, SAA3P, SLC6A9, SPN, TDGF1, TSC22D1 and HDAC5, the second set consists of CD178, MCP-1, TNF-alpha, IL- 1beta, IL-6, IL-10, INF-alpha and INF-gamma. A multilayered perceptron neural network analysis may be used for high accuracy prediction. A diagnostic means may be prepared from the method.