METHOD OF FORMING CALIBRATION CURVE FOR QUANTITATION ASSAY IN INFRARED SPECTROSCOPY AND QUANTITY DETERMINATION METHOD

摘要

PROBLEM TO BE SOLVED: To obtain a highly reliable calibration curve for a quantitation assay in infrared spectroscopy.;SOLUTION: The method comprises a preliminary solution preparation step of preparing two or more preliminary solutions 10 with different known contents of a solute dissolved in a solvent, a preliminary sample preparation step of supplying each preliminary solution 10 to a sample stage 20 and then removing the solvent to remain the solute on the stage 20, a preliminary spectroscopy step of irradiating the solute 11 remaining on the stage with infrared light through a bottom surface of the sample stage and taking an infrared absorption spectrum of the reflected light from the solute 11, and a calibration curve formation step of obtaining a reciprocal functionality between the known content of the solute in each preliminary solution and specific information of the solute in the infrared absorption spectrum. The sample stage 20 has a sample space 22 to receive the preliminary solution, and the bottom surface 23 of the sample space 22 irradiated with the infrared light is constructed from an infrared light-transmissive material. Each preliminary solution 10 is supplied to the sample space 22 so as to extend over the entire bottom surface of the sample space 22.;COPYRIGHT: (C)2010,JPO&INPIT