The use of hemodynamic endothelium / smooth muscle cell co-culture model in vitro to identify new therapeutic targets in vascular disease

摘要

Biomechanical in vitro model used to apply to human / animal cells in culture (ie, blood) hemodynamic modeled on the human circulation pattern. This model reproduces the hemodynamic flow patterns which are measured directly from the circulation of a person using a non-invasive magnetic resonance imaging, is converted to a motor which controls the rotation of the cone. Immersed fluid (ie, the cell culture medium) in, the cone is brought close to the surface of the cells growing in the plate surface. Rotation of the cone, creating a shear stress to convert the momentum fluid on the time-varying to the cell surface or plate. Mimics most closely the force hemodynamic physiological given to (cells lining the blood vessels) endothelial cells in vivo, this model is limited to the application of non-physiological flow patterns that are more simplified I overcome the flow devices ever has been. Another aspect of the present invention is directed to it to capture the Transwell co-culture dishes. It makes it possible to physically separate culture dishes environment in different cell types or more 2 to 3, the cell surface of the inner, which is exposed to hemodynamic flow pattern simulated while that. Inlet pipe of custom for supplying independent and separately to both the inner and the outer chamber of the co-culture model, medium, the drug, and the like, and important modifications of the other, including the outflow pipe. External components, is used to control the gas density and physiological temperature. Physical separation of adherent cells by the bottom of the Petri dish and transwell membrane artificial, can be isolated separately for (ie, protein, gene, etc.) of the biological analysis of a series, or surface cell layer of each I want to enable.