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E. coli has been metabolically engineered for enhanced production of sialic acid

机译：大肠杆菌经过代谢工程改造，可提高唾液酸的产量

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摘要

E. coli has been metabolically engineered to produce sialic acid method of making the stock and coli strains. E. coli has been operated in E. coli cell lines, nanT (sialic acid Adoraze) gene and is inactivated nanA nanT and (sialic acid transporter) gene ^- nanA ^- E. in E. coli cells and is overexpressed neuB gene and gene neuC of sialic acid biosynthesis in Neisseria meningitidisB group and is introduced. In addition, E. glucosamine synthase gene coli, glmS is concomitant overexpression with neuC and neuB.

机译：大肠杆菌已经被代谢工程化以产生唾液酸的方法来制备原种和大肠杆菌菌株。大肠杆菌已在大肠杆菌细胞系nanT（唾液酸Adoraze）基因中操作，并且已失活nanA nanT和（唾液酸转运蛋白）基因^{- nanA ^{- E并在大肠杆菌细胞中过量表达了neuB基因和脑膜炎奈瑟氏球菌B组中唾液酸生物合成的基因neuC。另外，大肠杆菌葡糖胺合酶基因glmS与neuC和neuB同时过表达。}}

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公开/公告号JP2010517509A

专利类型
公开/公告日2010-05-27

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申请/专利权人シラキュースユニバーシティ;
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申请/专利号JP20090530570
发明设计人ボディー;クリストファー;エヌ;ランドグレン;ベンジャミン;アール;
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申请日2007-09-26
分类号C12N1/21;C12N1/15;C12N5/10;C12N1/19;C12P7/40;C12N15/09;
国家 JP
入库时间 2022-08-21 19:02:15

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6. Succinic acid production from sucrose and molasses by metabolically engineered E. coli using a cell surface display system [J] . Jiangfeng Ma, Feng Li, Rongming Liu Biochemical Engineering Journal . 2014,第Null期

7. GENETICALLY ENGINEERED PROBIOTIC E. COLI NISSLE TO CONSUME AMINO ACIDS ASSOCIATED WITH ORPHAN METABOLIC DISEASES [C] . Ning Li, David Lubkowicz, Vincent Isabella, Microbial engineering . 2018

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9. Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from Metabolically-Engineered E. coli [O] . Francisco Aguilar, Thomas Scheper, Sascha Beutel 2019

10. 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Vascular304Mitophagy acts as a safeguard mechanism against human vascular smooth muscle cell apoptosis induced by atherogenic lipidsTranscriptional control and RNA species - Vascular307MicroRNA-34a role in vascular calcification308Local delivery of a miR-146a inhibitor utilizing a clinically applicable approach attenuates neointima formation after vascular injury309Long noncoding RNA landscape of hypoxic endothelial cells310Specific circulating microRNAs levels associate with hypertension, hyperglycemia and dysfunctional HDL in acute coronary syndrome patientsCytokines and cellular inflammation - Vascular313Phosphodiesterase5A up-regulation in vascular endothelium under pro-inflammatory conditions: a newly disclosed anti-inflammatory activity for the omega-3polyunsaturated aatty acid docosahexaenoic acid314Cardiovascular risk modifying with extra-low dose anticytokine drugs in rhematoid arthritis315Conversion of human M-CSF macrophages into foam cells reduces their proinflammatory responses to classical M1-polarizing activation316Lymphocytic myocarditis coincides with increased plaque inflammation and plaque hemorrhage in coronary arteries, facilitating myocardial infarction317Serum osteoprotegerin level predictsdeclined numerous of circulating endothelial- derived and mononuclear-derived progenitor cells in patients with metabolic syndromeGrowth factors and neurohormones - Vascular320Effect of gastrin-releasing peptide (GRP) on vascular inflammationSignal transduction - Heart323A new synthetic peptide regulates hypertrophy in vitro through means of the inhibition of nfkb324Inducible fibroblast-specific knockout of p38 alpha map kinase is cardioprotective in a mouse model of isoproterenol-induced cardiac hypertrophy325Regulation of beta-adrenoceptor-evoked inotropic responses by inhibitory G protein, adenylyl cyclase isoforms 5 and 6 and phosphodiesterases326Binding to RGS3 and stimulation of M2 muscarinic acetylcholine receptors modulates the substrate specificity of p190RhoGAP in cardiac myocytes327Cardiac regulation of post-translational modifications, parylation and deacetylation in LMNA dilated cardiomyopathy mouse model328Beta-adrenergic regulation of the b56delta/pp2a holoenzyme in cardiac myocytes through b56delta phosphorylation at serine 573Nitric oxide and reactive oxygen species - Vascular331Oxidative stress-induced miR-200c disrupts the regulatory loop among SIRT1, FOXO1 and eNOS332Antioxidant therapy prevents oxidative stress-induced endothelial dysfunction and Enhances Wound Healing333Morphological and biochemical characterization of red blood cell in coronary artery diseaseCytoskeleton and mechanotransduction - Heart336Novel myosin activator, JSH compounds, increased myocardial contractility without chronotropic effect in ratsExtracellular matrix and fibrosis - Vascular339Ablation of Toll-like receptor 9 causes cardiac rupture after myocardial infarction by attenuating proliferation and differentiation of cardiac fibroblasts340Altered vascular remodeling in the mouse hind limb ischemia model in Factor VII activating protease (FSAP) deficiencyVasculogenesis, angiogenesis and arteriogenesis343Pro-angiogenic effects of proly-hydroxylase inhibitors and their potential for use in a novel strategy of therapeutic angiogenesis for coronary total occlusion344Nrf2 drives angiogenesis in transcription-independent manner: new function of the master regulator of oxidative stress response345Angiogenic gene therapy, despite efficient vascular growth, is not able to improve muscle function in normoxic or chronically ischemic rabbit hindlimbs -role of capillary arterialization and shunting346Effect of PAR-1 inhibition on collateral vessel growth in the murine hind limb model347Quaking is a key regulator of endothelial cell differentiation, neovascularization and angiogenesis348"Emerging angiogenesis" in the chick chorioallantoic membrane (CAM). 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