首页> 外国专利> METHOD FOR PRODUCING RAW SILK FROM EXTRANEOUS-GENE-EXPRESSED BOMBYX MORI COCOON, AND PRODUCT USING THE RAW SILK

METHOD FOR PRODUCING RAW SILK FROM EXTRANEOUS-GENE-EXPRESSED BOMBYX MORI COCOON, AND PRODUCT USING THE RAW SILK

机译:用外源表达的Bombyx Mori茧生产生丝的方法及使用生丝的产品

摘要

PROBLEM TO BE SOLVED: To provide a method for producing raw silk from the cocoons of Bombyx mori with an expressed extraneous gene without impairing the properties of extraneous protein(s) therein.;SOLUTION: The method includes the following process: Cocoons are dried at a relatively low temperature of 60°C rather than dried at a 100°C or higher condition as is the case with normal production of raw silk from cocoons. Also, in boiling the cocoons, they are put to low-temperature and vacuum infiltration treatment (reduced-pressure treatment) rather than a conventional high-temperature treatment at 100°C or higher or a high-temperature treatment at near the boiling point. Also, as a cocoon-boiling method differing from the above, the cocoons are put to a combinational treatment of sericin swelling by alkali and hot-water infiltration into the cocoon layer by a surfactant, an enzyme or the like. Further, a silk reeling is conducted at a temperature lower than those in conventional cases. As a result, it has been found that the cocoons of Bombyx mori with an expressed extraneous gene can be made into raw silk without impairing the properties of extraneous protein(s) therein.;COPYRIGHT: (C)2010,JPO&INPIT
机译:要解决的问题:提供一种从家蚕的茧中表达出无关基因的生丝的方法,而又不损害其中的无关蛋白质的性质。解决方案:该方法包括以下过程:将茧干燥相对较低的温度为60℃,而不是像正常情况下从茧生产蚕丝那样在100℃或更高的条件下干燥。而且,在煮茧时,将它们进行低温和真空渗透处理(减压处理),而不是常规的在100℃或更高的高温处理或在接近沸点的高温处理。另外,作为不同于上述的茧煮方法,将茧通过碱和酶等通过碱与热水渗透到茧层中的组合进行丝胶蛋白溶胀的组合处理。此外,在比常规情况低的温度下进行silk丝。结果,已经发现具有表达的外源基因的家蚕茧可以制成生丝,而不会损害其中的外源蛋白的性质。;版权所有:(C)2010,JPO&INPIT

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