Screening method of the HBV multiplication deterrent which is based on cell imaging law, uses the interaction with HBV kapushidotanpaku and the surface protein

摘要

This invention, HBV (Hepatitis B Virus) interaction between kapushidotanpaku and the surface protein which are necessary for multiplication (degree of coupling) measuring with cell imaging, with the method the screening of doing the multiplication deterrent of HBV, in detail, is something regarding the method of measuring the change of cell imaging PreS domain and cell membrane target conversion functional PH of the surface protein of HBV (Pleckstrin homology) fusion protein, said fusion protein and operate mutually kapushidotanpaku and the fluorescent protein which include the arrangement of domain (GFP) due to the interaction between the fusion protein which is included. Interaction between the protein which is necessary for the HBV multiplication of this invention using the method the screening of doing at cell level, can the multiplication deterrent of new HBV use effectively to the screening at cell level.