Pyrroloquinoline quinone with excellent stability or substrate specificity (PQQ)-dependent glucose dehydrogenase variants

摘要

PROBLEM TO BE SOLVED: To provide a modified pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQGDH) with superior substrate specificity and stability.;SOLUTION: The modified pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQGDH) having lower activity with respect to disaccharides and/or greater stability than wild-type PQQGDH are provided. The gene of the enzyme, the vector, the method for producing the PQQGDH by the transformant to which the gene is introduced, the glucose sensor utilizing the gene, and the method for measuring the glucose are also provided.;COPYRIGHT: (C)2008,JPO&INPIT