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Detection method using N- nucleotide primer set for detecting the genotype of the acetyltransferase 2 (NAT2), kits, and the primer set

机译:使用N-核苷酸引物组检测乙酰转移酶2(NAT2)基因型的检测方法,试剂盒和引物组

摘要

Disclosed is easy in a short period of time the single nucleotide polymorphism of the NAT2 gene, to provide a method capable of and inexpensively detected, it is an object thereof is to provide a nucleic acid primer and a detection probe for use in said detection. For detecting a genotype of T341C single nucleotide polymorphism NAT2 gene [SOLUTION] person, there is provided a nucleotide primer set for LAMP amplification, used, and FIP primer selected from primer sets 1 to 10 listed in Table 5 consists of sequence of SEQ ID NO: 39 when the BIP primer, BIP primer and the FIP primer is selected from the primer sets 10 and 1 to 5 of Table 5, BIP primers and the FIP primer of primer sets 6-9 of Table 5 F3 primer having the sequence of SEQ ID NO: 41 when it is selected, and, to provide a nucleotide primer set and a B3 primer having the sequence of SEQ ID NO: 40. [Selection] Figure Figure 1
机译:本发明的目的是提供一种能够以低廉的价格检测到NAT2基因的单核苷酸多态性的方法,其目的是提供一种用于所述检测的核酸引物和检测探针。为了检测T341C单核苷酸多态性NAT2基因的基因型[SOLUTION]人,提供了用于LAMP扩增的核苷酸引物组,使用的是选自表5中所列引物组1-10的FIP引物,其由SEQ ID NO的序列组成。 :39:当从表5的引物组10和1至5中选择BIP引物,BIP引物和FIP引物时,表5的BIP引物和引物组6-9的FIP引物具有SEQ ID NO:1的序列。选择ID ID:41时,并提供核苷酸引物组和具有SEQ ID NO:40序列的B3引物。[选择]图1

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