After cleavage sulfur deprotection for the convergence of protein synthesis by chemical linking

摘要

The present invention provides methods and compositions for the synthesis of polypeptides or oligopeptides by convergent assembly of the oligonucleotide pairs in a chemical ligation reaction. A significant aspect of the present invention, it is an oligopeptide with which can spontaneously generate the free C-terminal thioester moiety is deprotected, a C-terminal disulfide protected carboxy thioester group. This allows for the single precursor is involved in the chemical coupling reaction sequence, whereby it becomes possible to convergent synthetic approach. Are useful in methods for the chemical synthesis of oligopeptides, polypeptides and, by using peptide fragments of 4 or more particularly, the present invention is to assemble a product of oligopeptides or polypeptides, In the case, I will improve the efficiency of chemical ligation of the native.