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Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection
Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection
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机译:无标记的转基因植物:无需使用抗生素选择即可改造叶绿体基因组
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摘要
The present invention provides for a method to circumvent the problem of using antibiotic resistant selectable markers. In particular, target plants are transformed using a plastid vector which contains heterologous DNA sequences coding for a phytotoxin detoxifying enzyme or protein. The selection process involves converting a antibiotic-free phytotoxic agent by the expressed phytotoxin detoxifying enzyme or protein to yield a nontoxic compound. The invention provides for various methods to use antibiotic-free selection in chloroplast transformation.
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