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METHODS OF MEASURING CONCENTRATION OF COMPONENT IN BIOCHEMICAL SAMPLE AND ESTIMATING RELIABILITY OF TEST RESULT

机译:测量生化样品中成分的浓度并评估测试结果可靠性的方法

摘要

Provided are a method of measuring a concentration of a component in a biochemical sample such as serum, and a method of estimating the reliability of a test result using the method. The method of measuring a concentration of a component includes: preparing plural standard samples having at least one common component which has varying concentrations; measuring absorbances of the component in the standard samples at each of the concentrations for plural light beams having different wavelength bands; determining a regression equation of a relationship between the concentration of the component and the measured absorbances at plural wavelength bands; and applying the regression equation by measuring absorbances at the plural wavelength bands of the biochemical sample and applying the measured plural absorbances of the biochemical sample to the regression equation to calculate the concentration of the component.
机译:提供了一种测量生化样品中诸如血清的成分的浓度的方法,以及使用该方法估计测试结果的可靠性的方法。测量组分浓度的方法包括:制备具有至少一种具有变化浓度的共同组分的多个标准样品;在具有不同波长带的多束光束的每个浓度下,测量标准样品中组分的吸光度;确定所述组分的浓度与在多个波长带处测得的吸光度之间的关系的回归方程;通过测量生化样品的多个波长带处的吸光度,并将测得的生化样品的多个吸光度应用于回归方程,来应用回归方程,以计算组分的浓度。

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