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Affinity peptides and method for purification of recombinant proteins
Affinity peptides and method for purification of recombinant proteins
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机译:亲和肽和纯化重组蛋白的方法
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摘要
This invention describes a process for separating a fusion protein or polypeptide in the form of its precursor from a mixture containing said fusion protein and impurities, which comprises contacting said fusion protein with a resin containing immobilized metal ions, said fusion protein covalently operably linked directly or indirectly to an immobilized metal ion-affinity peptide, binding said fusion protein to said resin, and selectively eluting said fusion protein from said resin.
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