Two-photon absorbing dipyrrometheneboron difluoride dyes and their applications

摘要

A separation free bioanalytical assay method for measuring an analyte from a biological fluid or suspension containing microparticles as a bioaffinity binding solid phase, a biospecific secondary reagent labelled with a two-photon fluorescent dipyrrometheneboron difluoride dye of specified structure, focusing the laser into the reaction suspension, and measuring two-photon excited fluorescence from single microparticles when they randomly float or are guided by the radiation pressure of the excitation laser through the focal volume of the laser beam.