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Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both PCR clamp and sensor probe
Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both PCR clamp and sensor probe
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机译:使用肽核酸同时作为PCR钳和传感器探针来检测核苷酸突变的方法和试剂盒
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摘要
Disclosed herein is a method for determining whether a target polynucleotide sequence contained in a nucleic acid sample has nucleotide variation(s) in a selected region thereof, the steps of which involve the use of a pair of primers that allows the formation of a PCR product having a sequence covering that of the selected region of the target polynucleotide sequence via a PCR process, and a peptide nucleic acid (PNA) that acts as a PCR clamp as well as a sensor probe. Also disclosed herein is a kit for use in determining the presence of nucleotide variation(s) in the target polynucleotide sequence, which includes the pair of primers and the PNA.
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