首页> 外国专利> Synthetic gene coding for human granulocyte-colony stimulating factor for the expression in E. coli

Synthetic gene coding for human granulocyte-colony stimulating factor for the expression in E. coli

机译：编码人类粒细胞集落刺激因子的合成基因，用于在 E中表达。大肠杆菌

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摘要

The invention relates to the synthetic gene coding for hG-CSF which enables expression in E. coli with an improved expression level of the recombinant hG-CSF regarding the total cellular proteins after expression. 展开▼

机译：本发明涉及编码hG-CSF的合成基因，其能够在I E中表达。重组人hG-CSF的表达水平相对于表达后的细胞总蛋白而言有所改善。展开▼

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公开/公告号US7655437B2

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公开/公告日2010-02-02

原文格式PDF

申请/专利权人 SIMONA JEVSEVAR;VIKTOR MENART;
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申请/专利号US20050522827

发明设计人 SIMONA JEVSEVAR;VIKTOR MENART;
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申请日2003-07-28

分类号C12N15;C12N15/11;C07K14;

国家 US

入库时间 2022-08-21 18:48:17

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1. 修饰的人类U1snRNA分子、编码修饰的人类U1snRNA分子的基因、包括该基因的表达载体及其在基因治疗中的用途 [P] . 中国专利： CN103314106B . 2017.09.12

2. 修饰的人类U1snRNA分子、编码修饰的人类U1snRNA分子的基因、包括该基因的表达载体及其在基因治疗中的用途 [P] . 中国专利： CN103314106A . 2013-09-18

3. SYNTHETIC GENE CODING FOR HUMAN GRANULOCYTE-COLONY STIMULATING FACTOR FOR THE EXPRESSION IN E. coli [P] . 欧洲知识产权局专利： EP1527095A1 . 2005-05-04

机译：大肠杆菌表达的人类粒细胞-结肠刺激因子的合成基因编码

4. SYNTHETIC GENE CODING FOR HUMAN GRANULOCYTE-COLONY STIMULATING FACTOR FOR THE EXPRESSION IN E. coli [P] . AU2003253344A1 . 2004-02-23

机译：大肠杆菌表达的人类粒细胞-结肠刺激因子的合成基因编码

5. SYNTHETIC GENE CODING FOR HUMAN GRANULOCYTE-COLONY STIMULATING FACTOR FOR THE EXPRESSION IN E. coli [P] . 世界知识产权组织专利： WO2004013175A1 . 2004-02-12

机译：大肠杆菌表达的人类粒细胞-结肠刺激因子的合成基因编码

1. Recombinant human granulocyte-colony stimulating factor and differential expression of cerebral cortical proteins in the subacute stage of cerebral ischemia/reperfusion [J] . Baohua Liu, Jing Dong, Lei Lu, 中国神经再生研究（英文版） . 2011,第019期

2. Recombinant human granulocyte-colony stimulating factor and differential expression of cerebral cortical proteins in the subacute stage of cerebral ischemia/reperfusion [J] . Baohua Liu, Jing Dong, Lei Lu, 中国神经再生研究：英文版 . 2011,第019期

3. Differential expression of bcl-2 and bax genes during the E. coli-induced apoptosis of human U937 cells [J] . JIA, HE, WANG, 中华微生物学和免疫学：英文版 . 2005,第004期

4. Subcloning and high level expression of xylE gene coding for the catechol 2, 3 dioxygenase in E. coli [J] . 蔡在龙, 王学敏, 毛积芳, 中国人民解放军军医大学学报：英文版 . 1997,第003期

5. Site-Directed Mutagenesis in Human Granulocyte-colony Stimulating Factor, Cloning and Expression in Escherichia coli [J] . Behzadian Farida, Ghorashi Seyed Ali, Naghoosi Hamed, Iranian Journal of Biotechnology . 2008,第4期

6. Enhancement of human granulocyte-colony stimulating factor production in recombinant E. coli using batch cultivation [J] . Valiollah Babaeipour, rnMahdi Pesaran Haji Abbas, Zahra Sahebnazar, Bioprocess and Biosystems Engineering . 2010,第5期

7. The effect of rare codons following the ATG start codon on expression of human granulocyte-colony stimulating factor in Escherichia coli [J] . Karimi Zeinab, Nezafat Navid, Negandaripour Manica, Protein Expression and Purification . 2015,第Null期

8. Exploring a Synthetic Proteome: Gl obal Analysis of Non-Genetically Encoded Amino Acid Incorporation Across the E. coli Proteome [C] . Hans Rudolf Aerni, Patrick ODonoghue, Svetlana Rogulina, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

9. I. Differential gene expression in human peripheral blood monocytes and alveolar macrophages. II. Macrophage colony-stimulating factor is important in the development of pulmonary fibrosis. [D] . Opalek, Judy Marcus. 2004

10. The expression in E. coli of synthetic repeating polymeric genes coding for poly(L-aspartyl-L-phenylalanine). [O] . M T Doel, M Eaton, E A Cook, 2006

11. Poster session 2Morphogenetic mechanisms290MiR-133 regulates retinoic acid pathway during early cardiac chamber specification291Bmp2 regulates atrial differentiation through miR-130 during early heart looping formationDevelopmental genetics294Association of deletion allele of insertion/deletion polymorphism in alpha 2B adrenoceptor gene and hypertension with or without type 2 diabetes mellitus295Association of G1359A polymorphism of the endocannabinoid type 1 receptor (CNR1) with coronary artery disease (CAD) with type 2 diabetes mellitusCell growth, differentiation and stem cells - Vascular298Gamma-secretase inhibitor prevents proliferation and migration of ductus arteriosus smooth muscle cells: a role of Notch signaling in postnatal closure of ductus arteriosus299Mesenchymal stromal-like cells (MLCs) derived from induced pluripotent stem (iPS) cells: a promising therapeutic option to promote neovascularization300Sonic Hedgehog promotes mesenchymal stem cell differentiation to vascular smooth muscle cells in cardiovacsular disease301Proinflammatory cytokine secretion and epigenetic modification in endothelial cells treated LPS-GinfivalisCell death and apoptosis - Vascular304Mitophagy acts as a safeguard mechanism against human vascular smooth muscle cell apoptosis induced by atherogenic lipidsTranscriptional control and RNA species - Vascular307MicroRNA-34a role in vascular calcification308Local delivery of a miR-146a inhibitor utilizing a clinically applicable approach attenuates neointima formation after vascular injury309Long noncoding RNA landscape of hypoxic endothelial cells310Specific circulating microRNAs levels associate with hypertension, hyperglycemia and dysfunctional HDL in acute coronary syndrome patientsCytokines and cellular inflammation - Vascular313Phosphodiesterase5A up-regulation in vascular endothelium under pro-inflammatory conditions: a newly disclosed anti-inflammatory activity for the omega-3polyunsaturated aatty acid docosahexaenoic acid314Cardiovascular risk modifying with extra-low dose anticytokine drugs in rhematoid arthritis315Conversion of human M-CSF macrophages into foam cells reduces their proinflammatory responses to classical M1-polarizing activation316Lymphocytic myocarditis coincides with increased plaque inflammation and plaque hemorrhage in coronary arteries, facilitating myocardial infarction317Serum osteoprotegerin level predictsdeclined numerous of circulating endothelial- derived and mononuclear-derived progenitor cells in patients with metabolic syndromeGrowth factors and neurohormones - Vascular320Effect of gastrin-releasing peptide (GRP) on vascular inflammationSignal transduction - Heart323A new synthetic peptide regulates hypertrophy in vitro through means of the inhibition of nfkb324Inducible fibroblast-specific knockout of p38 alpha map kinase is cardioprotective in a mouse model of isoproterenol-induced cardiac hypertrophy325Regulation of beta-adrenoceptor-evoked inotropic responses by inhibitory G protein, adenylyl cyclase isoforms 5 and 6 and phosphodiesterases326Binding to RGS3 and stimulation of M2 muscarinic acetylcholine receptors modulates the substrate specificity of p190RhoGAP in cardiac myocytes327Cardiac regulation of post-translational modifications, parylation and deacetylation in LMNA dilated cardiomyopathy mouse model328Beta-adrenergic regulation of the b56delta/pp2a holoenzyme in cardiac myocytes through b56delta phosphorylation at serine 573Nitric oxide and reactive oxygen species - Vascular331Oxidative stress-induced miR-200c disrupts the regulatory loop among SIRT1, FOXO1 and eNOS332Antioxidant therapy prevents oxidative stress-induced endothelial dysfunction and Enhances Wound Healing333Morphological and biochemical characterization of red blood cell in coronary artery diseaseCytoskeleton and mechanotransduction - Heart336Novel myosin activator, JSH compounds, increased myocardial contractility without chronotropic effect in ratsExtracellular matrix and fibrosis - Vascular339Ablation of Toll-like receptor 9 causes cardiac rupture after myocardial infarction by attenuating proliferation and differentiation of cardiac fibroblasts340Altered vascular remodeling in the mouse hind limb ischemia model in Factor VII activating protease (FSAP) deficiencyVasculogenesis, angiogenesis and arteriogenesis343Pro-angiogenic effects of proly-hydroxylase inhibitors and their potential for use in a novel strategy of therapeutic angiogenesis for coronary total occlusion344Nrf2 drives angiogenesis in transcription-independent manner: new function of the master regulator of oxidative stress response345Angiogenic gene therapy, despite efficient vascular growth, is not able to improve muscle function in normoxic or chronically ischemic rabbit hindlimbs -role of capillary arterialization and shunting346Effect of PAR-1 inhibition on collateral vessel growth in the murine hind limb model347Quaking is a key regulator of endothelial cell differentiation, neovascularization and angiogenesis348"Emerging angiogenesis" in the chick chorioallantoic membrane (CAM). 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V Garcia-Martinez, C Lopez Sanchez, W Hamed, 2016

1. Construction of recombinant plasmid and prokaryotic expression in E. Coli and biological activity analysis of human placenta arresten gene [J] . Jin-Ping Zheng Hai-Ying Tang Xian-Jiu Chen Bao-Feng Yu Jun Xie and Tang-Chun Wu Department of Toxicology (and Department of Biochemistry and Molecular Biology Shanxi Medical University Taiyuan 030001 China: Institute of Occupational Medicine Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China . 国际肝胆胰疾病杂志：英文版 . 2006,第1期

2. 甜菜坏死黄脉病毒内蒙古分离物RNA3序列分析及编码25ku蛋白基因在大肠杆菌中的表达 [C] . 李大伟 ,于嘉林 ,韩成贵 . 中国植物病理学会第六届代表大会暨学术年会 . 1998

3. 大鼠TSHβ基因编码DNA序列原核表达载体的构建及其在大肠杆菌中的表达和纯化 [A] . 刘玉冰 . 2007