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Conditional knockout method for gene trapping and gene targeting using an inducible gene silencer
Conditional knockout method for gene trapping and gene targeting using an inducible gene silencer
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机译:使用诱导型基因沉默子进行基因捕获和基因靶向的条件敲除方法
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摘要
A method for conditionally knocking out and altering gene function and genetic sequences that can be used in such methods, for use in gene trapping and gene targeting. Specifically, the genetic sequence is a inducible gene silencer comprising: (a) a splice acceptor sequence; (b) an internal ribosomal entry site (IRES) sequence; (c) a nucleotide sequence coding for a reporter protein; (d) a polyadenylation sequence; and (e) a pair of oppositely oriented recombination site sequences, which cause single cycle inversions in the presence of a suitable recombinase enzyme, flanking elements (a) through (d).
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