Method for producing ethanol from Starch using a Composition of Enzymes that occur with Strain Aspergillus phoenicis

摘要

Claim 1: a method to produce ethanol, characterized in that it comprises: (1) provide a mass of fermentation, the PH is adjusted to between 3.5 and 4.0; (2) mixing the Dough fermentation with Composition of (i) an enzyme that occurs with the strain Aspergillus phoenicis deposited as NRRL 50090,(JI) and a Yeast; and (3) incubate for a period of time of fermentation at temperature between 20 and 40u00f8c to produce ethanol.Claim 1: a method for making a Composition of Enzyme, characterized in that it comprises: (a) providing a substrate in Solid State; (b) cultivate the strain of Aspergillus phoenicis deposited as NRRL 50090 on the substrate during a First period of time on a cu00a0mara cultivation pa A Composition of Ra producing Enzyme.Claim 16 wherein the composition comprises: a Strain Aspergillus phoenicis deposited as NRRL 50090.Claim 1: a method for Adapting a fungus to produce Amylase Hydrolyze Starch that is optimal for a low pHCharacterized in that it comprises: (a) an original Strain of fungus inoculated in a Culture Medium with an initial pH value; (b) select a First Progeny strain that has been adapted to develop the first pH value; (c) the first Progeny Strain inoculated in a Culture Medium With a Second pH valueWhere the second pH value is less than the first pH value; (d) selecting a Second Strain of Progeny that is adapted to develop the second pH value; (e) optionally Repeat Steps (c) and (d) until the second pH value is the final value of pH (f); and Conclusion if the strain was obtained in STEP (e) is capable of producing a Composition of EnzymeHidrolizaru00a0 Starch at a pH lower than 5.