Stabilizing Composition of nucleic acids for Conservation at 4 degrees Celsius for prolonged periods

摘要

The present Invention relates to a composition for Stabilizing Nucleic Acids (deoxyribonucleic acid and ribonucleic acid RNA - DNA) in 4SC, for periods of more than a year, without affecting its Integrity.The solution stabilizes Nucleic Acids contents in their natural environment such as cells or tissues, serum, plasma, semen, saliva or other Biological Fluids, as well as Nucleic Acids Purified from these natural means, or Fragments of DNA / RNA obtained by su00edntensis Chemistry, Transcription in vitro (in the case of RNA) or other means.The sample can be preserved in the composition to 4SC, in Higher volumes to the volume necessary for analysis, which allows its reuse. This is very appropriate from the practical Point of view and in particular for Diagnostic purposes, for the Conservation of RNA / DNA Fragments.The Transportation and storage of the Quantification and internal standards used in the control of inhibitions and Tube to tube variations in nucleic acid amplification tests, either homemade or commercial, is more economical to 4SC.It is also a Feasible solution to conservation in the stabilizing 4SC, Nucleic Acid in their natural environment, during transportation to the laboratory and until the time of analysis.The composition described in the Patent is compatible with most Methods of isolation of nucleic acids and the most known RNA / DNA recovered from the same useful in demanding applications in Molecular Biology.