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A NEW METHOD FOR QUALITATIVE AND QUANTITATIVE DETECTION OF SHORT NUCLEIC ACID SEQUENCES OF ABOUT 8-50 NUCLEOTIDES IN LENGTH
A NEW METHOD FOR QUALITATIVE AND QUANTITATIVE DETECTION OF SHORT NUCLEIC ACID SEQUENCES OF ABOUT 8-50 NUCLEOTIDES IN LENGTH
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机译:定性和定量检测长约8-50个核苷酸的短核酸序列的新方法
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摘要
The present invention concerns a new analytical method for qualitative and quantitative detection of short nucleic acid sequences, preferably a DNA oligonucleotide or a modified DNA oligonucleotide such as antisense oligonucleotides or fragmented nucleic acid sequences of about 8-50 nucleotides in length, from especially biological sample material. The invention relates to the introduction of modified nucleic acids into an oligonucleotide probe that hybridizes to the target sequence such that amplification and quantitation of the short nucleic acid sequence is enabled and sensitivity and specificity of the reaction is increased. The invention also embraces test kits for performing nucleic acid amplification to detect and quantitate short nucleic acid sequences and processes for preparing such and the use of new analytical methods.
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