METHOD AND COMPOSITION FOR PREVENTING PHENOL POLLUTION AND INDUCING AXILLARY SHOOTS DEVELOPEMNT IN SHOOT APEX CULTURE OF SUGARCANE

摘要

The invention is related to a novel and simple method of shoot apex culture in sugarcane which prevents the phenolic excretion from the cut end of the shoot apex and accelerates effective growth of axillary buds during in vitro shoot multiplication. This improved method carrying out the steps of a simple surface sterilization and excision of shoot apex, a simple and modified method of inoculation to prevent phenol pollution and induction of axillary bud sprouting from the shoot apex in a modified culture medium.